GLAD (GLycan Array Dashboard)

Load Session

Choose session file to load:

Data Manipulations Performed

Choose Colors
Data Color

Auto Switch Data Colors
Selections
Sample Colors and Names will change only for Stage 2.
To change name click on the name and press enter when finished.
Stage 1
Filter Data:

e.g. "GalNAc,Gala" (no spaces)

Filter by Cut Off

Top % binders
Keep data for all samples if above cutoff for even single sample
Perform sort by id after filtering based on cutoff

Filter by Rank

Top Rank
Rank Order:
Keep data for all samples if within top rank for even single sample
Perform sort by id after filtering based on cutoff
Sort Data Order:

e.g. "GalNAc,Gala" (no spaces)

Normalize to value
Stage 2

Common Settings

Display Error Bars

Grouped Bar Charts Settings

Group bars by Name
Sort by Name
Grid Size
Value : Color
:
:
:
:
:
Force Range
Min
Max

(Below which no links are formed)

OR
Auto Find Maximum
Ignore Non-Binders
Force Strength
Between -1 to -100
-1 is closer and -100 more spread
Link Distance
between 10-100
Correlation Between:
Limits of Agreement SD
Value : Color
:
:
:
:
:
Choose Box, Line and Violin Color
OR
Use Sample Colors

Violin Settings

 
Custom Size:
Width: (min: 500)
Height: (min: 300)
 
Choose p-value cutoff:
 
Custom Y-Axis
Lower Limit: RFU
Upper Limit: RFU
Move Mouse Over Data to Draw Structures Below
Settings:
ms Delay Time for Drawing
Symbol Size

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